Journal: Nature Communications

Article Title: Inhibiting CD36 palmitoylation improves cardiac function post-infarction by regulating lipid metabolic homeostasis and autophagy

doi: 10.1038/s41467-025-61875-y

Figure Lengend Snippet: a qRT-PCR for quantifying the expression of mitochondrial-related genes between Sham and MI. Beta actin served as a reference gene. n = 8. b Myocardial ATP content in mice. n = 9. c Western blotting analysis of OXPHOS complexes in mitochondria of mouse cardiac tissue. Data are representative of six independent experiments. d , e Analyses of myocardial ceramide ( d ) and diacylglycerol (DAG) levels ( e ) in Sham and MI mice. n = 5. f , g Analyses of myocardial malondialdehyde (MDA) ( f ) and superoxide dismutase (SOD) ( g ) levels. n = 12 (MDA), n = 7 (SOD). Statistical significance was assessed by a two-tailed unpaired Student t test. h Changes in the expression of myocardial metabolism-related genes expression. Beta-actin as a reference gene. n = 6 (CPT1 and DGAT1), n = 9 (CPT2) and n = 10 (DGAT2 and PGC1α). i Representative 18 F-FDG images with microPET in the coronal and axial planes in Sham and MI mice 4 weeks post-LAD. Data are representative of five independent experiments. j Total protein level and plasma membrane distribution of Glut4 in cardiac tissue, as determined by Western blot analysis. n = 4. Data are presented as means ± SEM. Statistical significance was assessed by a two-tailed unpaired Student t test ( a , b , d – h and j ). Source data are provided as a file.

Article Snippet: Mitochondria were purified following the user protocol in the mitochondria extraction kit (KTP4003, KTP4004, Abbkine, Wuhan, China).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Two Tailed Test, Clinical Proteomics, Membrane

Journal: Nature Communications

Article Title: Inhibiting CD36 palmitoylation improves cardiac function post-infarction by regulating lipid metabolic homeostasis and autophagy

doi: 10.1038/s41467-025-61875-y

Figure Lengend Snippet: a Western blotting analysis of LC3II and SQSTM1 protein levels in myocardium from WT-CD36 and AA-SS-CD36 mice. n = 7. b Western blotting analysis of LC3II and SQSTM1 protein levels in cardiomyocytes transfected with WT-CD36 or AA-SS-CD36 for 48 h. n = 10. c Overlap between CD36 binding proteins, as assessed by CoIP-MS analysis and proteins in the Autophagy Database ( http://autophagy.info/ ). d Endogenous CD36 immunoprecipitation followed by anti-CD36 and anti-PGAM5 western blot analysis of cardiomyocytes (NMVCs). n = 4. e Quantification of Cyto-ID staining in cardiomyocytes (NMVCs) transfected with WT-CD36 or AA-SS-CD36 for 48 h, and then treated with 500 nM rapamycin (Rapa) and 60 µM CQ, the combination for 16 h. n = 6 per group. f – h Western blotting analysis of the mitochondria expression of CD36 and PGAM5 in cardiomyocytes (NMVCs) transfected with WT-CD36 or AA-SS-CD36. n = 7 per group. i Relative PGAM5 mRNA levels in cardiomyocytes transfected with WT-CD36 or AA-SS-CD36 for 48 h. n = 5. j Time course of PGAM5 protein stability in cardiomyocytes (NMVCs) transfected with WT-CD36 or AA-SS-CD36 after treatment with CHX (10 µg/ml). n = 6. k Ubiquitination of exogenous PGAM5 in cardiomyocytes (NMVCs) transfected with WT-CD36 or AA-SS-CD36. Data are representative of six independent experiments. l The mitochondria expression of phosphorylation Fundc1 and total Fundc1 protein in cardiomyocytes (NMVCs) transfected with WT-CD36 or AA-SS-CD36. n = 6 for phosphorylation Fundc1; n = 7 for total Fundc1. Data are presented as means ± SEM. Statistical significance was assessed by Kruskal-Wallis, followed by false discovery rate [FDR] method of Benjamini and Hochberg test ( a ), two-tailed unpaired Student t test ( b , d , g , i and l ), two-way ANOVA, followed by Tukey post hoc multicomparisons test ( e , j ) and two-tailed Mann-Whitney U test ( h ). Source data are provided as a file.

Article Snippet: Mitochondria were purified following the user protocol in the mitochondria extraction kit (KTP4003, KTP4004, Abbkine, Wuhan, China).

Techniques: Western Blot, Transfection, Binding Assay, Immunoprecipitation, Staining, Expressing, Ubiquitin Proteomics, Phospho-proteomics, Two Tailed Test, MANN-WHITNEY